Glucose mimics cell penetration

Ischemia-reperfusion causes severe neuronal damages in the process of stroke and other neurological diseases [ 1 , 2 ]. Oxygen and glucose deprivation OGD insult, followed by re-oxygenation OGDR , is applied in cultured neuronal cells to mimic the pathological condition of ischemia-reperfusion injuries [ 3 — 6 ]. Recent studies have suggested that OGDR mainly induces necrosis, but not apoptosis, in neuronal cells [ 5 , 7 , 8 ]. Necrosis is traditionally known as a passive cell death behavior.

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Development of a Cell-penetrating Peptide that Exhibits Responsive Changes in its Secondary Structure in the Cellular Environment

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Brain-vascular interactions and calcium signaling View all 6 Articles. The blood-brain barrier BBB , made up of endothelial cells of capillaries in the brain, maintains the microenvironment of the central nervous system. During ischemia and traumatic brain injury TBI , cellular disruption leading to mechanical insult results to the BBB being compromised. Oxygen glucose deprivation OGD is the most commonly used in vitro model for ischemia. On the other hand, stretch injury is currently being used to model TBI in vitro. In this paper, the two methods are used alone or in combination, to assess their effects on cerebrovascular endothelial cells cEND in the presence or absence of astrocytic factors. Damage to the cells induced release of lactate dehydrogenase enzyme LDH and nitric oxide NO into the cell culture medium.
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CNRS Technologies

An international team of researchers has developed miniscule, self-propelled devices that mimic the way cells move. The submicron-size swimmers borrow a page from larger microorganisms that can detect nutrients and toxins in their environment, and move toward or away from them by a process called chemotaxis. Neurons deploy these same mechanisms to migrate through the developing brain.
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Thanks to our expertise, our network and our know-how of the innovation ecosystem, we support you throughout your project. Peptide , Vector , Cell penetration , Constrained oligomers. The transport of substances through the plasma membrane and their access to intracellular compartments is a major problem in many therapies anticancer, antiviral, for example. Peptide vectors referred to as cell-penetrating peptides CPPs have emerged as promising tools for the intracellular delivery of a large set of molecules drugs, peptides, siRNA. However, the stability of these peptide compounds towards proteases involves a risk of rapid destruction of the conjugate vector-active molecule in vivo.
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